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CONFOCAL MICROSCOPE

Enhanced imaging of fluorescently labeled specimens using a Leica TCS SP8 MP scanning confocal microscope
 

Lasers

  • 405 nm for Dapi, Hoechst, CFP etc.
  • 488 nm for FITC, Alexa Fluor 488, GFP etc.
  • 552 nm for rhodamine, Texas red, mRFP1, Alexa Fluor 546, 555, 568, 594 etc.
  • 633 nmfor Cy5 etc.
  • Spectra-Physics Mai Tai DeepSee tunable ultrafast laser (680 - 1040 nm) for multi-photon excitation

Microscope

Leica TCS SP8 MP confocal microscope, with the following objectives

  • HC PL Fluotar 10x 0.3 n.a.
  • HC PL Apochromat 20x 0.75 n.a.
  • IRAPO 25x 0.95 n.a. dipping lens for live cell and in-vivo imaging
  • HC PL Apochromat 40x 1.30 n.a. oil
  • IRAPO 40x 1.10 n.a. Corr water immersion
  • HC PL Apochromat 63x 1.4 n.a. oil

System Resources 

  • Imaging of most standard fluorophores, in either single-photon or multi-photon modes.
  • Simultaneous fluorescent and DIC imaging of most samples
  • Heated, motorized stage for live cell imaging.
    • Real time measurements of cell migration, ion flux and metabolic profiling (NADH, FP)
  • Spectral imaging with up to 81 narrow wavelength bands (5 nm) over 380-785 nm for spectra-based experiments such as:
    • Separation of fluorophores with closely-spaced emission peaks by linear un-mixing
    • Generation of fluorophore-specific spectra
    • Background subtraction
    • Intensity based measurements of protein-protein interactions in live and fixed samples using FRET techniques
  • Fluorescence correlation spectroscopy (FCS).
    • Study of molecular dynamics in living cells
    • Generation of diffusion coefficients
  • Time-correlated single photo counting (TCSPC) using a Becker & Hickl spectroscopy system.
    • Lifetime-based measurements of protein-protein interactions in fixed and living samples using FRET/FLIM techniques
  • Software for FRET, FRAP and FLIM applications, ratiometric imaging, and complex time series experiments.

Additional Equipment

  • Total Internal Reflection Fluorscence (TIRF) imaging on an inverted Olympus microscope (488 nm excitation).
    • Single molecule bleaching and observations
    • High axial resolution renderings of the features and events on the plasma membrane in living cells
We strive to provide dynamic imaging services and training for researchers and students including; experimental design, image acquisition, analysis (including statistics and figure generation) and potential installation of specialized equipment to meet each investigators research needs.

Contact us.