Creighton University

TECHNIQUE AND IMAGE GALLERY

• What is confocal microscopy?
• How to make pictures and movies with depth!
• How to use the automated stage to make tiled images.
• How to show two fluorescently labeled proteins are ‘co-localized.’
• Förster resonance energy transfer techniques yield information about functional distances between proteins.
• Generate emission spectra for individual fluorophores which can be used for separation of overlapping spectra, background reduction and enhanced multicolored imaging.
• Quantification of observed differences in fluorescently labeled samples. 
• Physiology experiments for measurement of cellular dynamics.
• Redox Imaging to measure changes in cellular metabolism
• Mg⁺ Green Imaging to determine changes in ATP levels
• Quantification of Ca⁺⁺ release from living cells.

What is Confocal Microscopy?

Visit Zeiss or Olympus for great confocal tutorials.

 

Projected and Stereoscopic Images

How to make pictures and movies with depth!

 

Large Area Imaging

How to use the automated stage to make tiled images.

 

Protien 'Co-Localization' (within 200nm)

How to show two fluorescently labeled protiens are 'co-localized.'

 

Protien Co-Localization (within 5 to 10 nm)

Förster resonance energy transfer (FRET) techniques yield information about functional distances between two protiens.

 

Fluorescence Spectra Generation

Generate emission spectra for individual fluorophores which can be used for separation of overlapping.

 

Quantitative Fluorescence

Quantification of observed differences in fluorescence intensity.

 

Physiology Experiments

Redox imaging of living cells.

 

Magnesium Green measurement of changes in ATP concentration in living cells.

Multiple probes can measure the production of reactive oxygen species (ROS) in living cells. These probes are fluorescent only when oxidized and are sensitive to a wide variety of ROS. LeAnn Tiede, Creighton University Dept. of Biomedical Sciences.

Quantification of fluctuations in Ca⁺⁺ release from living cells.

 

Multicolor Imaging (Labeling of Cochlear Neurons Using 6 Different PTIR® Dyes)

Dr. Bernd Fritzsch, University of Iowa, Department of Biology, Dr. Michael Nichols, Creighton University Department of Biomedical Sciences and Physics.

 

3D Reconstruction of a Staphylococcus Aureus Biofilm

Ethan Mann, University of Nebraska Medical Center, Department of Microbiology and Immunology.

 

Alpha-actinin Labeling in Smooth Muscle Cells

 Dr. Yan Xie, Creighton University Pharmacology

 

Nile Red Stained Snake Skin

Laxmi Fogueri and Dr. Somnath Singh, Creighton University Pharmacy

 

Convallaria Autofluorescence

Dr. Heather Jensen-Smith, Creighton University Department of Biomedical Sciences

 

 Afferent and Efferent Innervation of the Cochlea

 Dr. Bernd Fritzsch, University of Iowa, Department of Biology

 

Nuclear Co-Localization of Poly A Binding Protien and hnPRC in Heat Shocked HeLa Cells

Korley Leland, Dana Salerno, Dr. Kristen Drescher, Creighton University Department of Medical Microbiology and Immunology

 

Streptoccus Pneumoniae Phagocytized by Alveolar Macrophages

Adam Pitz, Dr. Martha Gentry-Nielsen, Creighton University Department of Medical Microbiology and Immunology

 

Cerebellum Crosssection

Sarah Hake, Dr. Annemarie Shibata, Creighton University Department of Biology