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Confocal Microscopy

     The Creighton University Integrated Biomedical Imaging Core Facility contains a multi-photon confocal microscope that allows high resolution imaging of labeled cell components in three dimensional space. The confocal microscope, available to investigators in the United States, is supported by a grant from the State of Nebraska LB692 program, administered by the Creighton University School of Medicine and from users like you. Establishment of the facility and operation for the first three years was supported by a grant from the National Science Foundation (EPS-0346476) via Nebraska-E.P.S.Co.R.

   The floor on which the facility resides was constructed with support from C06 Grant RR17417-01 from the National Center for Research Resources of the National Institutes of Health. The facility, located in Criss II room 405, has been instrumental in the creation of forums for scientific interaction among Nebraska cell biologists, including an annual symposium on imaging methods, and has increased outreach to the undergraduate universities of Nebraska by providing summer fellowships for students and faculty to work with Center faculty.

   The Core is equipped with a Zeiss LSM 510 META NLO confocal scanning system consisting of an Argon 458/477/488/514 nm laser, a Green HeNE 543 nm, a Red HeNe 633 nm laser, and a Coherent ULTRA near infrared tunable Titanium Sapphire laser (690 - 1040 nm). Other facility equipment includes the Zeiss AxioPlan 2IE MOT motorized upright microscope, a META scanning module with 32 narrow wavelength band single channel detectors, two non-descanned detectors for multi-photon work, a Becker & Hickl spectroscopy system for fluorescence correlation spectroscopy (FCS) and time-correlated single photon counting (TCSPC), as well as a motorized stage for time series experiments.

   Imaging can be done with most standard fluorophores, in either single-photon or multi-photon modes. The META multi-channel system enables separation of fluorophores with closely-spaced emission peaks by linear un-mixing. Software is available for FRET, FRAP and FLIM, ratiometric imaging, and complex time series experiments. In addition to the confocal scanning system, the Core has metamorph 3-D reconstruction on a Nikon Eclipse 800, and high-resolution transmitted light and epifluorescence imaging on a Zeiss Axioskop II with 100x Plan Apochromat objective and SPOT RT camera as well as a TIRF microscope.

Key Personnel

Richard Hallworth, Ph.D., Director,