About the Typhoon
The Typhoon 9410 is an advanced gel and blot imaging system that enables imaging using storage phosphor autoradiography technology, as well as direct fluorescent or chemiluminescent imaging, for such applications as immunoblotting (Western blotting), Southern hybridization, and scanning slides of samples stained with labeled antibodies. This instrument was purchased in 2010 with support from an NIH shared instrumentation grant (1S10RR027352-01) primarily to enable NIH funded-researchers at Creighton to better accomplish their research goals, while also enhancing access to state-of-the-art technology to other Creighton investigators to improve research competitiveness overall. The instrument is located in Criss II, room 422. Access to this facility is restricted, but permission may be granted by contacting the facility director, Dr. Patrick Swanson. For further information about instrument capabilities, access, or scheduling, please click on the specific links on the left
- Versatile system platform, handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and even microarrays.
- Powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets.
- Red (633 nm)-, green (532 nm) -, and blue (457, 488 nm) -excitation wavelengths and a wide choice of emission filters enable imaging of an extensive variety of fluorophores.
- Five orders of magnitude (100 000:1) with less than 7.5% relative standard deviation for entire dynamic range.
- Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increasing throughput, and saving time.
- Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.
- Highly sensitive optics enable direct chemiluminescent imaging without intermediate exposure to films or screens.
- Provides optimized detection of Cy2, Cy3, and Cy5 CyDye DIGE Fluor minimal and saturation dyes, enabling visualization of up to three differently labeled samples on a single gel.
- Exceptional signal-to-noise increases the number of statistically significant differences detected.
- Consistent point-light illumination produces high-quality uniform images and reduces photo-bleaching.
- Gels are scanned between glass plates, preventing drying and shrinkage and allowing further running and rescanning if required.
- Analysis using ImageQuantTL ; offers four separate functional areas within one product:
Ø 1-D electrophoresis gels.
Ø Dot blots, microplates, and basic arrays.
Ø Colony counting and measurement of 2-D spots and other image features.
Ø Toolbox to analyze gels with user-defined objects.
For further information on the Typhoon 9410, see: http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=166013
For further information on the ImageQuantTL analysis software, see:
Access and Scheduling
The Typhoon 9410 is located in Criss II, room 422. Access to this corridor and room are restricted. Access may be granted by contacting Dr. Patrick Swanson (ph. 280-2716; email: firstname.lastname@example.org). Instrument time will be scheduled using the Calendar function in Microsoft Outlook. This calendar is named “TyphoonImaging” and permission to access the calendar may be granted by contacting Dr. Swanson. You will be able to establish and edit your own appointments, but not edit those made by others. Usage will be monitored and any potential conflicts will be addressed by an oversight committee chaired by Dr. Swanson. Because the equipment was purchased with NIH funds, NIH-funded investigators will receive highest priority to use the instrument if usage becomes heavy.