Patrick C. Swanson, PhD
Patrick C. Swanson, PhD

Patrick C. Swanson, PhD

Professor
Program Director,
School of Medicine

Academic Appointments

Department

  • Med. Microbiology & Immunology

Position

  • Professor

Biography

Examples of the most recent publications can be obtained from this PubMed link.

Education

  • St. Olaf College,BA - Chemistry,9/86 - 6/90
  • University of Michigan,Ph.D. - Chemistry,9/90 - 8/95

Publications and Presentations

Articles

  • Haney SL, Upchurch GM, Opavska J, Klinkebiel D, Hlady RA, Suresh A, Pirruccello SJ, Shukla V, Lu R, Costinean S, Rizzino A, Karpf AR, Joshi S, Swanson P, Opavsky R.  Promoter Hypomethylation and Expression Is Conserved in Mouse Chronic Lymphocytic Leukemia Induced by Decreased or Inactivated Dnmt3a., Cell Reports, 15(6), 1190-201, 2016
  • Shukla A, Cutucache CE, Sutton GL, Pitner MA, Rai K, Rai S, Opavsky R, Swanson PC, Joshi SS.  Absence of caveolin-1 leads to delayed development of chronic lymphocytic leukemia in Eμ-TCL1 mouse model., Experimental hematology, 44(1), 30-7, 2016
  • Palmer VL, Nganga VK, Rothermund ME, Perry GA, Swanson PC.  Cd1d regulates B cell development but not B cell accumulation and IL10 production in mice with pathologic CD5(+) B cell expansion., BMC Immunology, 16, 66, 2015
  • Palmer VL, Aziz-Seible R, Kassmeier MD, Rothermund M, Perry GA, Swanson PC.  VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection., Journal of Immunology, 195(4), 1524-37, 2015
  • Swanson P.C., V(D)J Recombination, Somatic Hypermutation, and Class Switch Recombination. In Reference Module in Biomedical Sciences. Elsevier. 18-Oct-2014 doi: 10.1016/B978-0-12-801238-3.00113-6 Michael J. Caplan (Ed. in Chief), Not Applicable, 2014
  • Manning J, Mitchell B, Appadurai DA, Shakya A, Pierce LJ, Wang H, Nganga V, Swanson PC, May JM, Tantin D, Spangrude GJ. Vitamin C promotes maturation of T-cells., Not Applicable, 19(17), 2013
  • Nakagawa T, Mondal K, Swanson PC. VprBP (DCAF1): a promiscuous substrate recognition subunit that incorporates into both RING-family CRL4 and HECT-family EDD/UBR5 E3 ubiquitin ligases., BMC Molecular Biology, 14, 22, 2013
  • Ma D, Li Y, Hackfort B, Zhao Y, Xiao J, Swanson PC, Lappe J, Xiao P, Cullen D, Akhter M, Recker R, Xiao GG. Smoke-induced signal molecules in bone marrow cells from altered low-density lipoprotein receptor-related protein 5 mice., Journal of Proteome Research, 11(7), 3548-3560, 2012
  • Wang G, Dhar K, Swanson PC, Levitus M, Chang Y. Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex., Nucleic Acids Research, 40(13), 6082-6096, 2012
  • AN Kriatchko, S Bergeron, and PC Swanson. ”HMG-box domain stimulation of RAG1/2 cleavage activity is metal ion dependent”, BMC Molecular Biology, 9:32, 2008
  • M Zhang and PC Swanson. ”V(D)J recombinase binding and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies”, J. Biol. Chem., 283(11), 6717-6127, 2008
  • P Raval, AN Kriatchko, S Kumar and PC Swanson. ”Evidence for Ku70/Ku80 association with full-length RAG1”, Nucleic Acids Research, 36 (6), 2060-2072, 2008
  • M Ciubotaru, AN Kriatchko, PC Swanson, FV Bright, and DG Schatz. ”Fluorescence resonance energy transfer analysis of recombination signal sequence configuration in the RAG1/2 synaptic complex”, Molecular and Cellular Biology, 27(13), 4745-4758, 2007
  • Raghavan SC, J Gu, PC Swanson, and MR Lieber. ”The structure-specific nicking of small heteroduplexes by the RAG complex: Implications for chromosomal translocations”, DNA Repair, 6, 751-759, 2007
  • Kriatchko, AN, DK Anderson, and PC Swanson. ”Identification and Characterization of a Gain-of-Function RAG-1 Mutant”, Molecular and Cellular Biology, Vol. 26, No. 12, 4712-4728, 2006
  • Bergeron S, DK Anderson, and PC Swanson ”RAG and HMGB1 Proteins: Purification and Biochemical Analysis of Recombination Signal Complexes”, Methods Enzymol., 408, 511-528, 2006
  • Bergeron, S, T Medathipirambil, and PC Swanson ”Both High Mobility Group (HMG)-boxes and the Acidic Tail of HMGB1 Regulate Recombination-Activating Gene (RAG)-mediated Recombination Signal Synapsis and Cleavage in vitro”, J Biol Chem, Vol. 280, No.35, 31314-31324, 2005
  • Raghavan, SC, PC Swanson, Y Ma, and MR Lieber. ”Double-Strand Break Formation by the RAG Complex at the Bcl-2 Major Breakpoint Region and at other non-B DNA Structures in vitro”, Molecular and Cellular Biology, Vol. 25, No 14, 5904-5919, 2005
  • The bounty of RAGs: recombination signal complexes and reaction outcomes, Immunological Reviews, 200, 90-114 , 2004
  • Raghavan SC, Swanson PC, Wu X, Hsieh C-L, and Lieber MR. ”A Non-B DNA structure at the bcl-2 major breakpoint region is cleaved by the RAG complex”, Nature, 428, 29-31, 2004
  • Swanson PC, Volkmer D, and Wang L. ”Full-length RAG-2, and not full-length RAG-1, specifically suppresses RAG-mediated transposition, but not hybrid joint formation or disintegration”, J Biol Chem, 279 (6), 4034-4044, 2004
  • Swanson, P.C. A RAG-1/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signals, Molecular and Cellular Biology, Vol. 22, issue 22, 7790-7801, 2002
  • ”B Cell Development” Chapter in Immunology for Medical Students by Nairn and Helbert, Not Applicable, 2002
  • P.C. Swanson,”Fine Structure and Activity of Discrete RAG-HMG Complexes on V(D)J Recombination Signals”, Molecular and Cellular Biology, 22, 1340-1351, 2002
  • Patrick C. Swanson ”The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination”, Molecular and Cellular Biology, Vol. 21, No. 2, 449-458, 2001
  • P.C. Swanson and S. Desiderio ”RAG-2 Promotes Heptamer Occu[ancy by RAG-1 in the Assembly of a V(D)J Initiation Complex Mol. Cell. Biol. 1999 19, 3674-3683, Not Applicable, 1999
  • P.C. Swanson.”A RAG-1-RAG-2 Tetramer Supports 12/23-Regulated Synapsis, Cleavage, and Transposition of V(D)J Recombination Signals”. Submitted to Immunity.
  • Swanson, P. Medical Microbiology and Immunology Review, 2002. In press at Biotest Publishing Co., Oxnard, CA.

Publications

  • Patrick C. Swanson, Dustin Volkmer, and Lei Wang ”The 'dispensable' regions of RAG-1 and RAG-2 play separable roles in regulating V(D)J recombinase activity in vitro”, FASEB Journal, 17, C190, 2003
  • Swanson, P.C. ”A RAG-1/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signals” Keystone Symposia, B cells and Antibodies, Keystone, Colorado, Not Applicable, 2003
  • Swanson, P.C. ”A molecular characterization of RAG-1/RAG-2 synaptic complexes involved in V(D)J recombination” 18th UICC International Cancer Congress Oslo, Norway, Not Applicable, 2002

Presentations

  • St. Albert's Day Poster Session, Creighton University. Poster Presentation. N. MAX SCHABLA, Greg A. Perry, James T. Fletcher, Patrick C. Swanson. "STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF VPRBP, A NOVEL RAG1-INTERACTING FACTOR", 2015
  • Allergy and Immunology Grand Rounds. Creighton University Omaha NE. Oral Presentation. Immunoglobulins: from genetics to B cell biology., 2015
  • 56th American Society for Hematology Annual Meeting and Exposition. San Francisco CA. Poster Presentation. Ashima Shukla, Christine E Cutucache, Karan Rai, Siddharth Rai, Rene Opavsky, Patrick Swanson, and Shantaram S Joshi. Absence of Caveloin-1 Leads to Delayed Development of Chronic Lymphocytic Leukemia in Eµ-TCL1 Mouse Model, 2014
  • Great Plains Analytical Cytometry Association 7th Annual Conference. Creighton University Omaha NE. Oral Presentation. "Using Multicolor Flow Cytometry And Cell Sorting To Define A Role For VprBP In B Cell Development And V(D)J Recombination"., 2014
  • Cold Spring Harbor Laboratories 2014 Meeting on GENE EXPRESSION & SIGNALING IN THE IMMUNE SYSTEM. Poster Presentation. Victoria L. Palmer, Razia Aziz-Seible, Mary Rothermund, Greg A. Perry, Patrick C. Swanson. VprBP, an E3 ubiquitin ligase adaptor protein that associates with full-length RAG1, is required for efficient V(D)J rearrangement of distal VH and Vk gene segments and editing of Igκ chain, but not for development of Igλ+ B cells, 2014
  • GCBA Seminar Series, UNMC, Omaha NE. Oral Presentation. Monoclonal B cell lymphocytosis and chronic lymphocytic leukemia: possible origin due to defects in B cell receptor editing?, 2014
  • Midwest Student Biomedical Research Forum, Omaha NE. Poster Presentation. Razia S. Aziz-Seible, Koushik Mondal, Vicky L. Palmer, Greg A. Perry and Patrick C. Swanson. Is Herc2 required for B cell development and V(D)J recombination?, 2013
  • School of Pharmacy Seminar Series, UNMC, Omaha NE. Oral Presentation "A RAG1-associating E3 ubiquitin ligase regulates B cell development and V(D)J recombination", 2012
  • CUSOM Faculty Club Reception, Omaha NE. S Kumar and PC Swanson. Poster Presentation. "Evidence for long-range RAG1/2 interactions with coding sequences in discrete V(D)J initiation complexes assembled on coupled cleavage substrates", 2008
  • 39th Annual Midwest Student Biomedical Research Forum, Omaha NE. AN Kriatchko, S Bergeron, and PC Swanson. Oral Presentation. "HMG-box domain stimulation of RAG1/2 cleavage activity is metal ion dependent", 2008
  • 39th Annual Midwest Student Biomedical Research Forum, Omaha NE. M Zhang, S Bergeron and PC Swanson. Poster Presentation. "HMGB1 promotes structure-specific DNA cleavage by the RAG proteins via a nick-hairpin mechanism", 2008
  • 39th Annual Midwest Student Biomedical Research Forum, Omaha NE. S Kumar and PC Swanson. Poster Presentation. "Identification of long range protein-DNA contacts in synaptic complexes involved in V(D)J recombination", 2008
  • Keystone Synposium "DNA Replication and Recombination" Santa Fe, NM Oral Presentation "Evidence for long-range RAG1/2 interactions with coding sequences in discrete V(D)J initiation complexes assembled on coupled cleavage substrates", 2008
  • Keystone Synposium "DNA Replication and Recombination" Santa Fe, NM. Poster Presentation. PC Swanson, M Zhang, and S Bergeron. "HMGB1 promotes structure-specific DNA cleavage by the RAG proteins via a nick-hairpin mechanism.", 2008
  • Keystone Synposium "DNA Replication and Recombination" Santa Fe, NM. Poster Presentation. S Kumar and PC Swanson "Evidence for long-range RAG1/2 interactions with coding sequences in discrete V(D)J initiation complexes assembled on coupled cleavage substrates", 2008
  • Molecular and Cellular Biology REsearch Group Oral Presentation "Linking DNA breaks to DNA repair in V(D)J recombination", 2007
  • CUSOM Innovation in Basic Science and Clinical Research, Joselyn Art Museum Omaha NE. P Raval, AN Kriatchko, and PC Swanson. Poster Presentation. "Evidence for Ku70/80 association with full-length RAG1", 2007
  • CUSOM Innovation in Basic Science and Clinical Research, Joselyn Art Museum Omaha NE. SK Anderson, AE Hassaballa, K Parks, GA Perry and PC Swanson. Poster Presentation. "Immunodeficiency and accumulation of B1-like B cells in transgenic mice overexpressing catalytically inactive RAG1", 2007
  • UNMC Department of Pathology/Microbiology and Center for Leukemia and Lymphoma Research. "Links between V(D)J recombination and lymphoid cell malignancy: mechanisms and models", 2007
  • 94th Annual Meeting of the American Association of Immunologists. Miami, FL. PC Swanson, DK Anderson, AE Hassaballa, K Parks, and GA Perry. Poster Presentation. "Immunodeficiency and accumulation of B1-like B cells in transgenic mice overexpressing catalytically inactive RAG1", 2007
  • 94th Annual Meeting of the American Association of Immunologists. Miami, FL. P Raval, S Kumar, AN Kriatchko, and PC Swanson. Poster Presentation. "Evidence for Ku70/Ku80 association with full-length RAG1", 2007
  • 94th Annual Meeting of the American Association of Immunologists. Miami, FL. M Zhang and PC Swanson. Poster Presentation. "Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies", 2007
  • St. Albert's Day Poster Session, Creighton University. M Zhang and PC Swanson. Poster Presentation. "Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies", 2007
  • St. Albert's Day Poster Session, Creighton University. AN Kriatchko, DK Anderson, and PC Swanson. Poster Presentation. "Identification of a gain-of-function RAG1 mutant", 2007
  • 3rd Annual Nebraska Research and Innovation Conference. Poster Presentation. PC Swanson and GA Perry. "The Creighton University Flow Cytometry Core Facility: Machines and methods for high speed cell analysis and cell sorting." Quest Center Omaha, NE, 2007
  • Oral Presentation "Molecular Science at Creighton University: A brief overview" Nebraska Biomedical Research Retreat, Quarry Oaks, NE, 2006
  • EPSCoR 2006 Expo, Lincoln, NE Business Display for Creighton University Flow Cytometry Core Facility Perry, GA and PC Swanson, 2006
  • ASM Conference "Mobile DNA" Banff, Alberta, Canada "Characterization of a Novel Gain-of-Function RAG-1 Mutant", 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE AE Hassaballa (graduate student) Oral Presentation "Characterization of transgenic mice expressing catalytically inactive recombination activating gene 1", 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE P Raval (postdoctoral fellow), S Kumar (postdoctoral fellow), and PC Swanson. Poster Presentation "Association of full-length RAG1 to Ku links the cleavage and joining phases of V(D)J recombination", 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE A Kriatchko (graduate student), DK Anderson, and PC Swanson Poster Presentation "Characterization of a novel gain-of-function RAG-1 mutant", 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE M Zhang (graduate student) and PC Swanson Poster Presentation "Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies", 2006
  • Creighton University Department of Medicine Research Conference Omaha, NE "Autoimmunity and Immunodeficiency: A New Model Provides Insights into the Role of RAG-1", 2005
  • Department of Medical Microbiology and Immunology Special Seminar Perry GA, and PC Swanson "Multicolor Cell Analysis and Cell Sorting Arrives at Creighton University", 2005
  • UNMC Department of Genetics, Cell Biology and Anatomy seminar series. Omaha, NE "B cell development in transgenic mice overexpressing catalytically inactive RAG-1: implications for receptor editing and B1 versus B2 cell fate determination", 2005
  • Oral Presentation Immunology Interest Group, University of Nebraska Medical Center "B cell development in transgenic mice expressing catalytically inactive RAG-1", 2005
  • Bergeron S, Madathipirambil T, and Swanson PC. "Dissecting the structure-function relationship of HMGB1 in the initiation of V(D)J recombination in vitro". Poster presentation. Keystone Symposia: B cell development, function, and disease. Steamboat Springs, CO, 2005
  • Hassaballa AE, Perry GA, Parks KW, Anderson D, and Swanson PC. "Accumulation of transitional B cells in transgenic mice expressing catalytically inactive RAG-1". Poster presentation. Keystone Symposia: B cell development, function, and disease. Steamboat Springs, CO, 2005
  • Kriatchko AN, and Swanson PC. "Identification of a gain-of-function RAG-1 mutant". Poster presentation. Keystone Symposia: B cell development, function, and disease. Steamboat Springs, CO, 2005
  • Oral Presentation Allergy Fellows Teaching Conference "Antibodies: structure, function, origins,and investigations therein", 2005
  • Introductory remarks American Cancer Society Relay for Life, Midtown, 2005
  • Oral Presentation, Third Annual Microbiology Meeting, University of Nebraska, Lincoln "Recent Research on the V(D)J Recombinase", 2004
  • INBRE program seminar, Creighton University, Omaha, Nebraska "Antibodies: structure, function and origins of diversity", 2004
  • Hassaballa AE, Wang L, and Swanson PC. "Investigation of the dominant negative effect of catalytically deficient mutant RAG-1 in vivo. Poster presentation. Great Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE, 2004
  • Kriatchko, A and Swanson, PC. "Identification of a gain-of-function RAG-1 mutant" Poster presentation. Greaat Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE., 2004
  • Bergeron, S and Swanson, PC. "Dissecting the stucture-function relationship of HMGB1 in the initiation of V(D)J recombination." Poster presentation. Great Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE., 2004
  • Raval, P and Swanson, PC. "P125 copurifies with DNA-PK and shares an immunoreactive domain with Artemis." Poster presentation. Great Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE., 2004
  • BRIN program seminar, Creighton University, Omaha, Nebraska "Antibodies: structure, function and origins of diversity", 2003
  • Poster presentation, AAI Immunology 2003, Denver, Colorado "The 'dispensable' regions of RAG-1 and RAG-2 play separable roles in regulating V(D)J recombinase activity in vitro", 2003
  • Oral presentation, AAI Immunology 2003, Denver, Colorado "The 'dispensable' regions of RAG-1 and RAG-2 play separable roles in regulating V(D)J recombinase activity in vitro", 2003
  • Oral Presentation, Nebraska Wesleyan University, Lincoln, Nebraska "V(D)J recombination and the origins of immune repertoire diversity", 2003
  • Oral Presentation, Nucleic Acids Interest Group, Mahoney State Park "A RAG1/RAG2 tetramer supports synapsis, cleavage, and transposition of V(D)J recombination signals", 2002
  • Oral Presentation Immunology Interest Group University of Nebraska Medical Center Omaha, Nebraska "Characterization of RAG complexes supporting synapsis, cleavage, and transposition of V(D)J recombination signals", 2002
  • Poster Presentation 18th UICC Internation Cancer Congress, Oslo, Norway "A molecular characterization of RAG-1/RAG-2 synaptic complexes involved in V(D)J recombination", 2002
  • Third Annual Meeting: Great Plains States Society for Molecular Biology and Genetics;"12/23-Regulated Synapis,Cleavage, and Transposition of V (D)J Recombination Signals is Supported by a RAG-1/2 Tetramer Complex" Abstract and Poster Presentation, 2002
  • Keystone Symposium on Molecular Mechanisms of DNA Replication and Recombination, Snowbird,UT"Comparison of catalytic activity and protein-DNA contacts in discrete RAG-RSS complexes lacking or containing HMG-1 or HMG-2" Abstract and Poster presentation, 2002
  • AAAS Southwest and Rocky Mountain Division Meeting, Creighton University, "Assembly and Function of V (D)J Initiation Complexes", 2001
  • Nucleic Acids Interest Group, Mahoney State Park "Assembly and function of V(D)J initiation complexes" Oral Presentation, 2001
  • American Cancer Society Relay for Life; Norfolk, Nebraska; Introductory remarks in opening ceremony on the importance of cancer research and brief description my ACS funded research, 2001
  • Great Plains States Society for Molecular Biology and Genetics "Incorporation of HMG-1 or HMG-2 into V(D)J recombination pre-initiation complexes assembled on 23-RSS substrates stabilizes protein-DNA contacts at the heptamer and correlates with enhanced DNA cleavage activity" Poster Presentation, 2001
  • AAAS SWARM Meeting "The V(D)J recombinase cleaves DNA in trans" Oral Presentation, 2000
  • Immunology Interest Group Meeting, UNMC "Roles of RAG-1 and RAG-2 in V(D)J recombination" Oral Presentation, 2000
  • Oklahoma Medical Research Foundation, Oklahoma City, OK "The roles of RAG-1 and RAG-2 in the recognition and cleavage of V(D)J recombination signals" Invited Presentation, 2000
  • Immunology 2000, Seattle, WA "The V(D)J recombinase cleaves DNA in trans" Poster and Oral Presentation, 2000
  • Carleton College, Northfield, MN "The assembly of the antigen receptor genes: how RAG-1 and RAG-2 initiate V(D)J recombination" Invited Presentation Poster and Oral Presentation, 2000
  • St. Olaf College, Northfield, MN "The assembly of the antigen receptor genes: how RAG-1 and RAG-2 initiate V(D)J recombination" Invited Presentation Poster and Oral Presentation, 2000
  • VA Hospital, 1999
  • Distinct Roles for RAG-1 and RAG-2 in V(D)J Recombination Signal Recognition and Initiation Complex Assembly Keystone Symposium on B Lymphocyte Biology and Disease February 1999, 1999

Research and Scholarship

Research and Scholarship Interests

  • I am generally interested in mechanisms of immune repertoire diversification.  My research program is currently focused on studying various aspects of V(D)J recombination, the process by which immunoglobulin and T cell receptor genes are assembled during lymphocyte development.  V(D)J recombination is initiated by the RAG1 and RAG2 proteins, which together introduce DNA breaks in the antigen receptor loci, which are subsequently repaired by components of the non-homologous DNA end-joining (NHEJ) repair pathway (Fig. 1).  The laboratory has two major ongoing projects.  The first project is primarily biochemical, and is broadly aimed at identifying and characterizing protein-DNA complexes involved in cleavage and repair phases of V(D)J recombination.  We are particularly interested in learning how the DNA ends produced by RAG cleavage are transferred to the NHEJ apparatus for repair.  We believe NHEJ is facilitated by the associated of NHEJ factors with pre-cleavage RAG complexes, as we have recently published evidence for the association of the Ku70/Ku80 with full-length RAG1.  The second project involves determining whether enforced expression of catalytically inactive RAG1 in mice can impair secondary V(D)J recombination events triggered by engagement of self-antigen (receptor editing) or in response to immunization (receptor revision), and whether defects in these processes increase susceptibility to malignancy or autoimmune disease.  These research projects are or have been supported by both intramural and extramural grants, including grants awarded by the National Institutes of Health and the American Cancer Society.

Current Research Projects

Awards and Honors

  • PCT - TRANSGENIC MICE EXPRESSING A UNIQUE B CELL POPULATION AND METHODS OF USE (Patent No: N/A), 2006
  • Intramural Research Award, 2004
  • Intramural Research Award, 2002
  • Intramural Research Award, 1999