Ronald A. Shikiya, PhD
Ronald A. Shikiya, PhD

Ronald A. Shikiya, PhD

Research Assistant Professor
School of Medicine

Academic Appointments

Department

  • Med. Microbiology & Immunology

Position

  • Research Assistant Professor

Education

  • University of Nebraska Medical Center,Ph.D.,1999-2005

Publications and Presentations

Books

  • Prions and Diseases Volume 1, Physiology and Pathophysiology Chapter: Prion Strain Interference Charles R. Schutt, Ronald A. Shikiya and Jason C. Bartz ISBN: 978-1-4614-5304-8 (Print) ISBN: 978-1-4614-5305-5 (Online), Not Applicable, Springer; 2013 edition, 193-204, 2012

Articles

  • J Virol. 2016 May 27;90(12):5715-23. doi: 10.1128/JVI.00409-16. Print 2016 Jun 15.
    Incongruity between Prion Conversion and Incubation Period following Coinfection.
    Langenfeld KA1, Shikiya RA1, Kincaid AE2, Bartz JC3., Journal of Virology, 90(12), 2016
  • Biochim Biophys Acta. 2016 May;1860(5):990-8. doi: 10.1016/j.bbagen.2015.10.002. Epub 2015 Oct 9.
    Application of differential scanning calorimetry to measure the differential binding of ions, water and protons in the unfolding of DNA molecules.
    Olsen CM1, Shikiya R1, Ganugula R1, Reiling-Steffensmeier C1, Khutsishvili I1, Johnson SE1, Marky LA2., Biochimica et Biophysica Acta, 1860(5), 2016
  • Melissa D. Clouse, Ronald A. Shikiya, Jason C. Bartz, Anthony E. Kincaid “Nasal Associated Lymphoid Tissue of the Syrian Golden Hamster Expresses High Levels of PrPC” PLoS One. 10(2) 2015. doi:10.1371/journal.pone.0117935., PLoS One, 10(2), 2015
  • Prion formation, but not clearance, is supported by protein misfolding cyclic amplification. Shikiya RA, Eckland TE, Young AJ, Bartz JC doi: 10.4161/19336896.2014.983759, Prion, 8(6), 2014
  • Ronald A. Shikiya, Thomas E. Eckland, Alan J. Young, and Jason C. Bartz. “Prion formation, but not clearance, is supported by protein misfolding cyclic amplification” Prion 8(6): 1-6. 2014. http://dx.doi.org/10.4161/19336896.2014.983759, Prion, 8(6), 2014
  • The Story of Prions’ Discovery Shikiya, Ronald A. doi: 10.1093/biosci/biu158, BioScience, 64(11), 1052-1053, 2014
  • Charles R. Schutt, Ronald A. Shikiya, Jason C. Bartz. “Prions and Diseases: Prion Strain Interference” Springer Science + Business Media. New York. 2013. ISBN: 9781461453048, Springer Publishing, 2013
  • Protein misfolding cyclic amplification of prions Saunders SE1, Bartz JC, Shikiya RA doi:10.3791/4075., Journal of Visualized Experiment, 69, pii: 4075, 2012
  • Samuel E. Saunders, Jason C. Bartz, and Ronald A. Shikiya. “Protein misfolding cyclic amplification of prions” Journal of Visualized Experiments. 2012 Nov 7; 69. pii: 4075. doi: 10.3791/4075., Journal of Visualized Experiment, 69, 2012
  • In vitro generation of high-titer prions. Shikiya RA, Bartz JC doi: 10.1128/JVI.06134-11, Journal of Virology, 85(24), 13439-42, 2011
  • Ronald A. Shikiya and Jason C. Bartz. “In Vitro Generation of High-Titer Prions” Journal of Virology. 2011. 85(24). 13439-13442. Doi: 10.1128/JVI.06134-11, Journal of Virology, 85(24), 2011
  • Replication efficiency of soil-bound prions varies with soil type. Saunders SE1, Shikiya RA, Langenfeld K, Bartelt-Hunt SL, Bartz JC doi: 10.1128/JVI.00282-11, Journal of Virology, 85(11), 5476-82, 2011
  • Replication efficiency of soil-bound prions varies with soil type. Saunders SE1, Shikiya RA, Langenfeld K, Bartelt-Hunt SL, Bartz JC doi: 10.1128/JVI.00282-11, Journal of Virology, 85(11), 5476-82, 2011
  • Samuel E. Saunders, Ronald A. Shikiya, Katie A. Langenfeld, Shannon L. Bartelt-Hunt, and Jason C. Bartz. “Replication Efficiency of Soil-Bound Prions varies with Soil Type” Journal of Virology. 2011. 85(11). 5476-5482. Doi: 10.1128/JVI.00282-11, Journal of Virology, 85(11), 5476-82, 2011
  • Jacob I. Ayers, Charles R. Schutt, Ronald A. Shikiya, Adriano Aguzzi, Anthony E. Kincaid, Jason C. Bartz. “The Strain-Encoded Relationship between PrPSc Replication, Stability and Processing in Neurons is Predictive of the Incubation Period of Disease” PLoS Pathogens. 2011. 7(3). e1001317., PLoS Pathogens, 7(3), 2011
  • The strain-encoded relationship between PrP replication, stability and processing in neurons is predictive of the incubation period of disease. Ayers JI, Schutt CR, Shikiya RA, Aguzzi A, Kincaid AE, Bartz JC doi: 10.1371/journal.ppat.1001317, PLoS Pathogens, 7(3), 2011
  • Coinfecting prion strains compete for a limiting cellular resource. Shikiya RA1, Ayers JI, Schutt CR, Kincaid AE, Bartz JC doi: 10.1128/JVI.00243-10, Journal of Virology, 84(11), 5706-14, 2010
  • Ronald A. Shikiya, Charles Schutt, Jacob Ayer, Anthony Kincaid, and Jason Bartz. “Co-infecting Prion Strains Compete for a Limiting Cellular Resource” The Journal of Virology. 2010. 84(11) 5706-5714. Doi: 10.1128/JVI.00243-10, Journal of Virology, 84(11), 2010
  • Luis A. Marky, Souvik Maiti, Chris Olsen, Ronald Shikiya, Sarah E. Johnson, Mahima Kaushik, Irine Khutsishvili. “Building Blocks of Nucleic Acid Nanostructures: Unfolding Thermodynamics of Intramolecular DNA Complexes” Biomedical Applications of Nanotechnology. John Wiley & Sons, Inc. 2007. ISBN: 9780471722427, John Wiley & Sons, Inc, 2007
  • Ana Maria Soto; Dionisios Rentzeperis; Ronald Shikiya; Manuel Alonso; Luis A. Marky. “DNA Intramolecular Triplexes Containing dT→dU Substitutions: Unfolding Energetics and Ligand Binding” Biochemistry. 2006. 45(9) 3051-3059, Biochemistry, 45(9), 2006
  • Ronald Shikiya and Luis A. Marky, “Calorimetric unfolding of intramolecular triplexes: length dependence and incorporation of single AT -> TA substitutions in the duplex domain.” J Phys Chem B. 2005. 109(38) 18177-18183, Journal of Physical Chemistry B, 109(38) , 2005
  • Ronald Shikiya, Jian-Sen Li, Barry Gold, and Luis A. Marky, “Incorporation of cationic chains in the Dickerson-Drew dodecamer: correlation of energetics, structure, and ion and water binding”. Biochemistry. 2005. 44(37) 12582-12588, Biochemistry, 44(37), 12582-12588, 2005
  • Jian-Sen Li, F. Cheng, Ronald Shikiya, Luis A. Marky, and Barry Gold. “Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIPs”. J. Am. Chem. Soc. 2005. 127(36) 12657-12665, Journal of American Chemical Society, 127(36) , 12657-12665, 2005
  • Luis A. Marky, Karen Alessi, Ronald Shikiya, Jian-Sen Li, and Barry Gold. “Folding of Bent Oligodeoxynucleotide Duplexes: Energetics, Ion Binding and Hydration”. Nucleic Acids: Curvature and Deformation. ACS Symposium Series Book Chapter. Oxford University Press. September 2004. ISBN: 9780841238626, ACS Symposium Book Series, 2004
  • Jian-Sen Li, Ronald Shikiya, Luis A. Marky, and Barry Gold. “Triple Helix Forming TRIPside Molecules That Target Mixed Purine/Pyrimidine DNA Sequences”. Biochemistry, 2004. 43(6) 1440-1448, Biochemistry, 43(6) , 1440-1448, 2004
  • Besik I. Kankia, Ana Maria Soto, Nicole Burns, Ronald Shikiya, Chang-Shung Tung, and Luis A. Marky. “DNA Oligonucleotide Duplexes Containing Intramolecular Platinated Cross-Links: Energetics, Hydration, Sequence, and Ionic Effects”. Biopolymers, 2002. 65(3). 218-227, Biopolymers, 65(3), 218-227, 2002
  • Dionisios Rentzeperis, Ronald Shikiya, Souvik Maiti, James Ho, and Luis A. Marky. “Folding of Intramolecular DNA Hairpin Loops: Enthalpy-Entropy Compensations and Hydration Contributions”. Journal of Physical Chemistry B, 2002. 106(38) 9945-9950, Journal of Physical Chemistry B, 106(38), 9945-9950, 2002

Publications

  • Restricted propagation of sheep scrapie in hamster
    http://dx.doi.org/10.1080/19336896.2016.1162644
     , Prion, 10(Sup1), 2016

Research and Scholarship

Grant Funding Received

  • Protein conformational dynamics as indicator of interspecies transmission in prion diseases