Anna M. Selmecki, PhD
Anna M. Selmecki, PhD

Anna M. Selmecki, PhD

Assistant Professor
School of Medicine

Expertise/Specializations

  • Genomics, Fungal Pathogens, Evolution, Mechanisms of Drug Resistance, Polyploidy and Aneuploidy

Academic Appointments

Department

  • Med. Microbiology & Immunology

Position

  • Assistant Professor

Biography

Examples of the most recent publications can be obtained from this PubMed link.

Publications and Presentations

Books

  • Todd RT, Forche A, Selmecki A. (2017)  Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution.  The Fungal Kingdom. Chapter 28. Edited by Joseph Heitman, Barbara Howlett, Pedro Crous, Eva Stukenbrock, Timothy James, and Neil Gow., American Society for Microbiology, ISBN-9781555819576, Chapter 28, 2017

Articles

  • Jelenić I., Selmecki A., Laan L., Pavin N.  Spindle Dynamics Model Explains Chromosome Loss Rates in Yeast Polyploid Cells. Frontiers in Genetics. Aug 6;9:296. doi: 10.3389/fgene.2018.00296. eCollection 2018. PMID: 30131823. PMCID: PMC6091489, Frontiers in Genetics, 9, 296, 2018
  • Todd R.T., Braverman A., Selmecki A. Flow Cytometry Analysis of Fungal Ploidy. Current Protocols in Microbiology .Aug;50(1):e58. doi: 10.1002/cpmc.58. Epub 2018 Jul 20. PMID: 30028911. PMCID: PMC6105418, Protocols in Microbiology, 50 (1), e58, 2018
  • Franke J., Bloomberg W., Todd R.T., Selmecki A. Assembly of a complete genome sequence for Gemmata obscuriglobusreveals a novel prokaryotic rRNA operon gene architecture. Antonie Van Leeuwenhoek. 2018 May 21. doi: 10.1007/s10482-018-1102-0.
     , Antonie Van Leeuwenhoek, 2018
  • Mount H., Revie N., Todd R.T., Anstett K., Collins C., Costanzo M., Boone C., Robbins N., Selmecki A., and Cowen L.E.  Analysis of Genetic Circuitry and Adaptive Mechanisms Enabling Resistance to the Azole Antifungal Drugs. PLoS Genetics. 14(4):e1007319. PMID: 29702647, PLoS Genetics, 14(4), 2018
  • Xie JL, Qin L, Miao Z, Grys BT, Diaz JC, Ting K, Krieger JR, Tong J, Tan K, Leach MD, Ketela T, Moran MF, Krysan DJ, Boone C, Andrews BJ, Selmecki A, Ho Wong K, Robbins N, Cowen LE. (2017)  The Candida albicans transcription factor Cas5 couples stress responses, drug resistance and cell cycle regulation.
    Nature Communications  8(1):499., Nature Communications, 8 (1), 2017
  • Scott A, Richmond P, Dowell D, Selmecki A. (2017) The influence of polyploidy on the evolution of yeast grown in a sub-optimal carbon source. Molecular Biology and Evolution., Molecular Biology and Evolution, 34 (10), 2690-2703, 2017
  • Todd RT, Forche A, Selmecki A. (2017)  Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution. Microbiology Spectrum. 5 (4)., Microbiology Spectrum, 5, 2017
  • Selmecki, A., Maruvka, Y., Richmond, P., Guillet, M., Shoresh, N., Sorenson, A., De, S., Kishony, R., Michor, F., Dowell, R., Pellman, D. Polyploidy can drive rapid adaptation in yeast., Nature, 519 (7543), 349-52, 2015

Presentations

  • The University of Iowa, Biology Department, Iowa City, IA, 2018
  • Mutation Rate Evolution Meeting, Arizona State University, AZ, 2018
  • Ferlic Scholars Poster Presentation, Creighton University, Tyler Wikoff, 2018
  • University of Nebraska at Lincoln, Biology Department, Lincoln, NE, 2018
  • Nebraska INBRE Annual meeting - Oral Presentation - 1st place award Tyler Rollman, 2018
  • Gordon Research Conference on Molecular & Cellular Fungal Biology, Holderness, NH, 2018
  • Gordon Research Seminar (GRS) and Gordon Research Conference (GRC) on Molecular & Cellular Fungal Biology, Holderness, NH - First Place Poster presentation award Robert Todd, 2018
  • Plenary Seminar, Gordon Research Seminar (GRS) on Molecular & Cellular Fungal Biology, Holderness, NH, 2018
  • 13thAmerican Society for Microbiology Conference on Candida and Candidiasis, Providence,RI , 2018
  • 13th American Society for Microbiology Conference on Candida and Candidiasis, Providence, RI
    Poster Presentation and ISHAM Travel Award to Robert Todd, 2018
  • American Society of Microbiology, Combined Meeting of the Missouri and Missouri Valley Branches, 2018
  • American Society of Microbiology, Combined Meeting of the Missouri and Missouri Valley Branches, Undergraduate Poster Presentation – 2nd Place Poster Presentation Award to Tyler Wikoff and Shilpa Nair, 2018
  • American Society of Microbiology, Combined Meeting of the Missouri and Missouri Valley Branches, Graduate Oral Presentation – 2nd Place Oral Presentation Award to Robert Todd, 2018
  • American Society of Microbiology, Combined Meeting of the Missouri and Missouri Valley Branches, Undergraduate Oral Presentation – 1st Place Oral Presentation Award to Alison Guyer, 2018
  • American Society of Microbiology, Combined Meeting of the Missouri and Missouri Valley Branches, Undergraduate Poster Presentation - Ann Braverman, 2018
  • Midwest Student Biomedical Research Forum, Oral Presentation - 4th Place Oral Presentation Award to Robert Todd, 2018
  • EMBO Comparative Genomics of Eukaryotic Microbes: Dissecting Sources of Evolutionary Diversity, Barcelona, Spain, 2017
  • Ferlic Scholars Poster Presentation, Creighton University - Shilpa Nair, 2017
  • Nebraska INBRE Annual meeting - Oral Presentation - 1st place award to Alison Guyer, 2017
  • Canadian Institute for Advanced Research, Whistler BC, Canada, 2017
  • Eppley Institute, University of Nebraska Medical Center, Omaha, NE, 2017
  • St. Alberts Day Oral Presentation -1st Place Oral Presentation Award to Robert Todd, 2017
  • St. Alberts Day Oral Presentation - Robert Thomas, 2017
  • CURAS Summer Research Poster Presentation - Ann Braverman, 2017
  • CURAS Summer Research Poster Presentation - Alison Guyer, 2017
  • Creighton University St. Albert’s Day Research Forum, Omaha, NE, 2017
  • Tel-Aviv University, Department of Biology, Israel, 2017
  • Weizmann Institute of Science, Israel, 2017
  • University of Nebraska Medical School, Department of Pathology and Microbiology, 2016
  • Creighton University School of Medicine, Department of Biomedical Sciences, Research Seminar, 2016
  • University of Iowa, Department of Biology, 2016
  • Iowa State University - Department of Ecology, Evolution, and Organismal Biology, 2016
  • University of Nebraska Medical Center, Department of Genetics, Cell Biology and Anatomy, 2016
  • University of Missouri Kansas City, Biology Department Research Seminar, Kansas City, MO, 2016
  • EMBO Conference on Experimental Approaches to Evolution and Ecology, Heidelberg Germany, 2016
  • University of Nebraska Omaha, Biology Department Faculty Seminar, 2016
  • ASM Conference on Microbial Evolution, 2016
  • INBRE Faculty Research Seminar for summer INBRE scholars, 2016
  • Gordon Research Conference on Cellular and Molecular Fungal Biology, New Hampshire, 2016
  • International Conference on Polyploidy and Hybridization, Rovinj Croatia, 2016
  • St. Alberts Day Poster Presentation - Alison Guyer, 2016
  • 13th ASM Conference on Candida and Candidiasis 2016, Seattle Washington, 2016
  • University of Nebraska Medical Center - Department of Pharmacy, 2016
  • Creighton University Medical Microbiology and Immunology Departmental Seminar, 2016
  • Rocky Moutain Yeast Meeting 2016 - Research Seminar, 2016
  • Rocky Moutain Yeast Meeting Poster Presentation - with trainees Kimberly Fischer, Ph. D. and Robert Thomas, 2016
  • Des Moines University - Research Seminar, 2015
  • Kavli Frontiers of Science - Poster Presentation - "The Impact of Polyploidy on the Rate and Dynamics of Adaptation", 2015
  • FASEB Conference on Molecular Pathogenesis, Keystone, CO, 2015
  • Poster Presentation - Gordon Conference on Molecular Mechanisms of Evolution, 2015
  • Nebraska Fungal Meeting, Lincoln, NE, 2015
  • Stowers Institute, Kansas City, MO, 2015
  • Worcester Polytechnic Institute, Worchester, MA, 2015

Research and Scholarship

Research and Scholarship Interests

  • Understanding the dynamics of how growth-promoting mutations arise and accumulate in a population of cells is a fundamental problem underlying our understanding of tumorigenesis, drug resistance, and the treatment of cancer. We use experimental evolution, mathematical modeling, and comparative genomics to understand the impact of mutations on adaptation of a cell and its surrounding population. 

    We recently optimized a flow cytometry-based system that enables us to detect the acquisition and spread of beneficial mutations within a population, and used this system to compare budding yeast strains that varied by genome copy number or ploidy (Selmecki et al., Nature 2015). We found that polyploid yeast adapted more rapidly than isogenic haploid or diploid yeast in raffinose medium, and that polyploid yeast acquired more mutations, including point mutations, large segmental aneuploidies, and whole chromosome aneuploidies. We will continue to use this system to study how changes in chromosome number (ploidy and aneuploidy), cell size, and environment affect genome stability and evolvability in fungal and mammalian cells. 

    Our previous research identified chromosome aneuploidy as a driver for the acquisition of antifungal drug resistance in the pathogenic yeast Candida albicans (Selmecki et al., Science 2006). We found that aneuploid cells arose within a population very rapidly in the presence of antifungal drug (Selmecki et al., PLoS Genetics 2009), and that increased copy number of two specific genes found on the most common aneuploid chromosome provided the drug resistance phenotype (Selmecki et al., Molecular Microbiology 2008). The mechanisms underlying this rapid genome restructuring are not known.

Current Research Projects

Grant Funding Received

  • Nebraska LB692 - Impact of Fluconazole on Genome Stability
  • Nebraska LB506 - Mechanisms of Genome Instability in Polyploid Cells
  • George Haddix President's Faculty Research - Biologically Collected Environment DNA for Water Quality and Public Health
  • Creighton University Global Initiative - Applying Genomics to the Elimination of River Blindness
  • NIH equipment supplement - Patrick Swanson
  • 2016 Nebraska Experimental Program to Stimulate Competitive Research (EPSCoR) Major Research Instrumentation grant for a Crieghton University High Performance Computer Cluster